Journal: Current protocols in cytometry

Article Title: Fluorescent Proteins for Flow Cytometry

doi: 10.1002/cpcy.17

Figure Lengend Snippet: Commonly Used Fluorescent Proteins

Article Snippet: Allele Biotechnology , mTFP1, mWasabi, mMaple, mClavGR2, pcFPs, LanYFP, LanRFP, and mNeonGreen , http://www.allelebiotech.com/fluorescent-proteins.

Techniques:

Journal: Current protocols in cytometry

Article Title: Fluorescent Proteins for Flow Cytometry

doi: 10.1002/cpcy.17

Figure Lengend Snippet: Non-Profit and Commercial Sources of Fluorescent Proteins and Expression Vectors

Article Snippet: Allele Biotechnology , mTFP1, mWasabi, mMaple, mClavGR2, pcFPs, LanYFP, LanRFP, and mNeonGreen , http://www.allelebiotech.com/fluorescent-proteins.

Techniques: Expressing, Plasmid Preparation, Recombinant, Luciferase, Selection, Marker

Journal: Genes to cells : devoted to molecular & cellular mechanisms

Article Title: Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1

doi: 10.1111/gtc.12125

Figure Lengend Snippet: FRET-FLIM analysis of mutant SOD1 in inclusions and cytosol during aggregation and disaggregation. (A) Pseudo-color fluorescence lifetime images of cells expressing both SOD1-G85R-mTFP1 and SOD1-G85R-cp173mVenus. White arrows indicate inclusions. Cells were treated for 16 h with no reagents (a), DMSO (b), or MG-132 (c). After MG-132 treatment for 16 h, cells were transferred to the recovery culture and incubated for 10 h (d and e). Images of cells without (d) or with (e) inclusion structures are shown. (B, C) Comparison of the fluorescence lifetime of the FRET donor SOD1-G85R-mTFP1 after MG-132 treatment for 8 h (B, lanes 2–5) or 16 h (B, lanes 6–8), or in recovery culture for 10 h (C, lanes 1–4). Fluorescence lifetime values of individual cells are shown as open circles (n = 10–20), and average values are shown with bars. Significant differences were determined using Student’s t-test: ★P < 0.01, ★★P < 0.05.

Article Snippet: Fluorescent protein sequences used for replacement included mGFP (monomeric A206K variant of EGFP) ( Zacharias et al. 2002 ), mPAGFP (monomeric A206K variant of PAGFP, which can be converted from a dark state to bright green fluorescent state by irradiation with violet light; kindly provided from Dr. Jennifer Lippincott-Schwartz, National Institutes of Health, Bethesda, MD) ( Patterson & Lippincott-Schwartz 2002 ), mTFP1 (a bright monomeric cyan fluorescent protein with a single-component fluorescence lifetime; Allele Biotechnology, San Diego, CA) ( Ai et al. 2006 ), mVenus (a bright monomeric yellow protein; kindly provided from Dr. Atsushi Miyawaki in RIKEN, Japan) ( Nagai et al. 2002 ), cp173mVenus (a circularly permutated mVenus, which exhibits altered rotational orientations between the fluorophores; kindly provided by Dr. Takeharu Nagai, Osaka University, Japan) ( Nagai et al. 2004 ), and TagRFP (a monomeric red fluorescent protein; Evrogen, Moscow, Russia) ( Merzlyak et al. 2007 ), yielding the expression vectors pTRE-SOD1-WT-mGFP, pTRE-SOD1-G85R-mGFP, pTRE-SOD1-G85R-mPAGFP, pTRE-SOD1-WT-mTFP1, pTRE-SOD1-G85R-mTFP1, pTRE-SOD1-WT-cp173mVenus, pTRE-SOD1-G85R-cp173mVenus, pTRE-SOD1-WT-mVenus, pTRE-SOD1-G85R-mVenus, pTRE-SOD1-WT-TagRFP, and pTRE-SOD1-G85R-TagRFP.

Techniques: Mutagenesis, Fluorescence, Expressing, Incubation, Comparison